UHPLC Determination of Naringin and Denatonium Benzoate in Children's Finger Paint Pads
摘 要
儿童指画印泥样品用甲醇与0.02 mol·L-1磷酸二氢钾溶液以体积比3∶7组成的混合液超声萃取20 min,冷却至室温,于16 000 r·min-1转速下离心15 min,取上清液过0.22 μm过滤膜过滤,采用超高效液相色谱法测定滤液中柚皮苷和苯甲酸地那铵的含量。以C18色谱柱为分离柱,用乙腈和pH 4.3的0.02 mol·L-1磷酸二氢钾溶液以不同比例混合的溶液为流动相进行梯度洗脱,用二极管阵列检测器测定。柚皮苷和苯甲酸地那铵的质量浓度均在0.1~5.0 mg·L-1内与其对应的峰面积呈线性关系,检出限(3S/N)依次为0.75,1.15 mg·kg-1。以空白样品为基体进行加标回收试验,所得回收率为73.5%~104%,测定值的相对标准偏差(n=6)为4.2%~6.6%。
Abstract
The sample of children's finger paint pad was extracted ultrasonically for 20 min with a mixture of methanol and 0.02 mol·L-1 KH2PO4 solution (volume ratio, 3:7). After cooling to room temperature,the solution was centrifugated for 15 min at the speed of 16 000 r·min-1. The supernatant was taken and filtered on 0.22 μm filtering membrane. UHPLC was applied to the determination of naringin and denatonium benzoate in the filtrate. C18 chromatographic column was used as separation column with mixtures of acetonitrile and 0.02 mol·L-1 KH2PO4 solution (pH 4.3) with different mixing ratios as mobile phase in the gradient elution, and diode array detector was adopted for determination. Linear relationships between values of peak area and mass concentration of naringin and denatonium benzoate were kept in the same range of 0.1-5.0 mg·L-1, with detection limits (3S/N) of 0.75, 1.15 mg·kg-1 respectively. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 73.5%-104%, with RSDs (n=6) of determined values in the range of 4.2%-6.6%.
中图分类号 O652.63 DOI 10.11973/lhjy-hx202002012
所属栏目 工作简报
基金项目
收稿日期 2019/6/21
修改稿日期
网络出版日期
作者单位点击查看
备注望秀丽,高级工程师,博士,研究方向为轻纺消费品中有毒有害物质的检测,wangxiuli19840603@163.com
引用该论文: WANG Xiuli,WEI Biwen,GAO Huan,YU Wenjia. UHPLC Determination of Naringin and Denatonium Benzoate in Children's Finger Paint Pads[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2020, 56(2): 191~195
望秀丽,卫碧文,高欢,于文佳. 超高效液相色谱法测定儿童指画印泥中柚皮苷和苯甲酸地那铵[J]. 理化检验-化学分册, 2020, 56(2): 191~195
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】RODGERS G C, TENENBEIN M. The role of aversive bittering agents in the prevention of pediatric poisonings[J]. Pediatrics, 1994,93(1):68-69.
【2】SIBERT J R, FRUDE N. Bittering agents in the prevention of accidental poisoning:Children's reactions to denatonium benzoate (Bitrex)[J]. Emergency Medicine Journal, 1991,8(1):1-7.
【3】HENDERSON M C, NEUMANN C M, BUHLER D R. Analysis of denatonium benzoate in oregon consumer products by HPLC[J]. Chemosphere, 1998,36(1):203-210.
【4】FAULKNER A, DEMONTIGNY P. High-performance liquid chromatographic determination of denatonium benzoate in ethanol with 5% polyvinylpyrrolidone[J]. Journal of Chromatography A, 1995,715(1):189-194.
【5】GADZAŁA-KOPCIUCH R M, BUSZEWSKA T, BUSZEWSKI B. Isolation and determination of denatonium benzoate in antifreezing agents by solid phase extraction and HPLC[J]. Journal of Liquid Chromatography & Related Technologies, 2000,23(20):3133-3142.
【6】黄文康,岳超,宋剑锋,等.HPLC同时测定衢枳壳中7种指标成分的含量[J].中国现代应用药学, 2018,35(3):404-407.
【7】WEON J B, LEE J, YUN B R, et al. Simultaneous determination of 14 bioactive compounds in Samchulkunbi-tang using HPLC-DAD and LC-MS[J]. Analytical Methods, 2014,6(15):6023-6030.
【8】袁志鹰,周晓倩,张梦通,等.TLC-HPLC-Q-TOF-MS法快速鉴定柑橘提取物消毒液中桔皮素、蜜橘黄素、新橙皮苷和柚皮苷及其含量测定[J].分析试验室, 2018,37(9):993-997.
【9】CAO J L, ZHOU S J, QIU F, et al. A simple and fast method for the simultaneous quantification of six flavonoids in Fructus aurantii by UPLC-PDA and confirmation by UPLC/ESI-Q-TOF-MS[J]. Analytical Methods, 2012,4(12):4121-4128.
【10】何冰倩,关旸,史冬玲,等.超高效液相色谱法测定不同产地白芷中6种香豆素类成分[J].理化检验-化学分册, 2019,55(5):602-606.
【2】SIBERT J R, FRUDE N. Bittering agents in the prevention of accidental poisoning:Children's reactions to denatonium benzoate (Bitrex)[J]. Emergency Medicine Journal, 1991,8(1):1-7.
【3】HENDERSON M C, NEUMANN C M, BUHLER D R. Analysis of denatonium benzoate in oregon consumer products by HPLC[J]. Chemosphere, 1998,36(1):203-210.
【4】FAULKNER A, DEMONTIGNY P. High-performance liquid chromatographic determination of denatonium benzoate in ethanol with 5% polyvinylpyrrolidone[J]. Journal of Chromatography A, 1995,715(1):189-194.
【5】GADZAŁA-KOPCIUCH R M, BUSZEWSKA T, BUSZEWSKI B. Isolation and determination of denatonium benzoate in antifreezing agents by solid phase extraction and HPLC[J]. Journal of Liquid Chromatography & Related Technologies, 2000,23(20):3133-3142.
【6】黄文康,岳超,宋剑锋,等.HPLC同时测定衢枳壳中7种指标成分的含量[J].中国现代应用药学, 2018,35(3):404-407.
【7】WEON J B, LEE J, YUN B R, et al. Simultaneous determination of 14 bioactive compounds in Samchulkunbi-tang using HPLC-DAD and LC-MS[J]. Analytical Methods, 2014,6(15):6023-6030.
【8】袁志鹰,周晓倩,张梦通,等.TLC-HPLC-Q-TOF-MS法快速鉴定柑橘提取物消毒液中桔皮素、蜜橘黄素、新橙皮苷和柚皮苷及其含量测定[J].分析试验室, 2018,37(9):993-997.
【9】CAO J L, ZHOU S J, QIU F, et al. A simple and fast method for the simultaneous quantification of six flavonoids in Fructus aurantii by UPLC-PDA and confirmation by UPLC/ESI-Q-TOF-MS[J]. Analytical Methods, 2012,4(12):4121-4128.
【10】何冰倩,关旸,史冬玲,等.超高效液相色谱法测定不同产地白芷中6种香豆素类成分[J].理化检验-化学分册, 2019,55(5):602-606.
相关信息