尿样以十六烷基三甲基溴化铵为辅助剂，正辛醇为萃取剂，萃取液离心后，取上层有机相，采用高效液相色谱法测定其中一氯苯的2种代谢物（4-氯邻苯二酚和对氯苯酚）的含量。以RD-C₁₈色谱柱为分离柱，以甲醇与0.15%（体积分数）的磷酸溶液以体积比65：35组成的混合液为流动相，在检测波长280 nm处进行测定。4-氯邻苯二酚和对氯苯酚的质量浓度均在600 μg·L^-1以内与其对应的峰面积呈线性关系，检出限（3S/N）为1.67，2.23 μg·L^-1，测定下限（10S/N）为5.55，7.43 μg·L^-1。以空白样品为基体进行加标回收试验，所得回收率为89.2%~97.4%，测定值的日内相对标准偏差（n=6）为2.1%~4.9%，日间相对标准偏差（n=18）为2.5%~5.3%。

The urine sample was extracted with n-octanol with hexadecyl trimethyl ammonium bromide as auxiliary solvent. The extract was centrifugated, and then the upper organic phase was taken. HPLC was applied to the determination of 2 metabolites (4-chlorocatechol and p-chlorophenol) of monochlorobenzene in the upper organic phase. RD-C₁₈ chromatographic column was used as separation column, and a mixture of methanol and 0.15% (φ) phosphoric acid solution with volume ratio of 65∶35 was used as mobile phase. Detection at 280 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of 4-chlorocatechol and p-chlorophenol were kept in the same range within 600 μg·L^-1, with detection limits (3S/N) of 1.67, 2.23 μg·L^-1 and lower limits of determination (10S/N) of 5.55, 7.43 μg·L^-1 respectively. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 89.2%-97.4%, with intra-day RSDs (n=6) and inter-day RSDs (n=18) of determined values in the ranges of 2.1%-4.9%, 2.5%-5.3%, respectively.