新鲜烟叶样品经0.4 mol·L^-1高氯酸溶液超声提取，以4 g·L^-1的丹磺酰氯丙酮溶液为衍生化试剂，在40℃下衍生40 min，采用高效液相色谱法测定样品溶液中腐胺、亚精胺、精胺等3种多胺的含量。以ZORBAX SB-C₁₈色谱柱（4.6 mm×150 mm，5 μm）为分离柱，用0.01 mol·L^-1乙酸铵溶液和乙腈以不同比例混合的溶液为流动相进行梯度洗脱，用二极管阵列检测器测定。3种多胺的质量浓度均在0.50~50.0 mg·L^-1内与其对应的峰面积呈线性关系，检出限（3S/N）为0.07~0.08 mg·L^-1，测定下限（10S/N）为0.23~0.28 mg·L^-1。方法用于新鲜烟叶样品的分析，加标回收率为86.4%~100%，测定值的相对标准偏差（n=6）为0.70%~4.4%。

The fresh tobacco leave sample was extracted ultrasonically by 0.4 mol·L^-1 HClO₄ solution, and then derived with using 4 g·L^-1 dansulfonyl chloride acetone solution as derivating reagent at 40℃ for 40 min. HPLC was applied to the determination of 3 polyamines, i.e. putrescine, spermidine and spermine in the sample solution. ZORBAX SB-C₁₈ chromatographic column (4.6 mm×150 mm, 5 μm) was used as separation column with mixtures of 0.01 mol·L^-1 ammonium acetate solution and acetonitrile with different mixing ratios as mobile phase in the gradient elution, and photodiode array detector was used for determination. Linear relationships between values of peak area and mass concentration of 3 polyamines were kept in the same range of 0.50-50.0 mg·L^-1, with detection limits (3S/N) in the range of 0.07-0.08 mg·L^-1 and lower limits of determination (10S/N) in the range of 0.23-0.28 mg·L^-1. The proposed method was applied to the analysis of samples of fresh tobacco leaves, giving values of recovery found by standard addition method and RSDs (n=6) of determined values in the ranges of 86.4%-100% and 0.70%-4.4% respectively.

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