高效液相色谱法测定烟草中烟碱含量
Determination of Nicotine in Tobacco by High Performance Liquid Chromatography
摘 要
建立了高效液相色谱法（HPLC）测定烟草中烟碱含量的方法。称取烟草样品粉末0.5 g，用含0.1 mol·L-1氢氧化钠的75%（体积分数）甲醇溶液溶解并定容至100 mL，超声提取30 min，采用HPLC进行测定。结果显示：烟碱的质量浓度在3.00~100 mg·L-1内与其对应的峰面积呈线性关系，检出限（3S/N）为0.5 mg·L-1；日内（n=6）和日间（n=9）测定值的相对标准偏差（RSD）分别为0.47%~1.1%和0.44%~0.97%；对烟草样品进行3个浓度水平的回收试验，烟碱的回收率为97.4%~100%。方法用于实际样品进行分析，并与气相色谱法（YC/T 246-2008）、分光光度法（GB/T 23225-2008）进行对比，所得测定值与分光光度法的测定值基本一致，并且所建立方法的测定值的RSD更小。
烟草
烟碱
超声提取
HPLC
tobacco
nicotine
ultrasonic extraction
Abstract
A method for the determination of nicotine in tobacco by high performance liquid chromatography (HPLC) was developed. Tobacco sample powder of 0.5 g was dissolved in 75% methanol solution containing 0.1 mol·L-1 sodium hydroxide and made its volume up to 100 mL. After ultrasonic extraction for 30 min, HPLC was used for determination. As shown by the results, linear relationship between mass concentration of nicotine and its peak area was kept in the range of 3.00-100 mg·L-1, with detection limit (3S/N) of 0.5 mg·L-1. RSDs of intraday (n=6) and interday (n=9) were 0.47%-1.1% and 0.44%-0.97%, respectively. Recovery test was made on tobacco sample at 3 concentration levels by standard addition method, giving results in the range of 97.4%-100%. This method was used to analyze the actual samples and compared with gas chromatography (YC/T 246-2008) and spectrophotometry (GB/T 23225-2008), with the determined values by this method basically consistent with those of spectrophotometry, and RSDs of the determined values by the proposed method were less.
中图分类号 O652.63 DOI 10.11973/lhjy-hx202110005
所属栏目 工作简报
基金项目
收稿日期 2020/6/30
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备注钱建瑞,工程师,硕士,主要从事植物提取物的成分分析与研究
引用该论文: QIAN Jianrui,YANG Qingshan,LI Xing,ZHANG Xiaofang,CHENG Yuanxin. Determination of Nicotine in Tobacco by High Performance Liquid Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2021, 57(10): 890～893
钱建瑞,杨清山,栗星,张晓芳,程远欣. 高效液相色谱法测定烟草中烟碱含量[J]. 理化检验－化学分册, 2021, 57(10): 890～893
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参考文献
【1】殷全玉, 杨铁钊, 郭宝银, 等.紫外分光光度法测定烟草中的游离烟碱[J].中国烟草科学, 2008, 29(6):20-22.
【2】张佳杰, 孟子晖, 薛敏, 等.烟碱样品前处理及分析方法研究进展[J].色谱, 2018, 36(11):1081-1087.
【3】谢笑天, 郑萍, 朱伟明, 等.高效液相色谱法测定卷烟烟气总粒相物中的烟碱[J].分析化学, 2000, 28(9):1085-1087.
【4】储志兵, 周新光, 水恒福, 等.反相高效液相色谱法测定烟草中烟碱的含量[J].农业环境科学学报, 2006, 25(增刊2):751-754.
【5】储志兵, 周新光, 水恒福, 等.超声提取-高效液相色谱法测定烟草中烟碱含量[J].化工技术与开发, 2007, 36(2):46-48.
【6】MANCEAU F, FLINIAUX M A, JACQUIN-DUBREUIL A. A high performance liquid chromatographic procedure for the analysis of tobacco alkaloids——Application to the evaluation of tobacco alkaloids in plants and cell suspension cultures[J]. Phytochemical Analysis, 1992, 3(2):65-68.
【7】LEE J H, PATRA J K, SHIN H S. Analytical methods for determination of carbonyl compounds and nicotine in electronic No-Smoking aid refill solutions[J]. Analytical Biochemistry, 2020, 588:113470.
【8】MOHAMMED Z H. Determination of nicotine extracted from eggplant and green pepper by HPLC[J]. Baghdad Science Journal, 2019, 16(1):61-67.
【9】廖坤, 彭春鸣, 张翼, 等.气相色谱法测定烟草中的烟碱含量[J].烟草科技, 2003, 36(3):32-34.
【10】蔡君兰, 陈黎, 彭斌, 等.气相色谱法同时测定电子烟烟液中的烟碱、1, 2-丙二醇和丙三醇[J].中国烟草学报, 2016, 22(5):1-9.
【11】YANG S, SMETENA I, HUANG C. Determination of tobacco alkaloids by gas chromatography with nitrogen-phosphorus detection[J]. Analytical and Bioanalytical Chemistry, 2002, 373(8):839-843.
【2】张佳杰, 孟子晖, 薛敏, 等.烟碱样品前处理及分析方法研究进展[J].色谱, 2018, 36(11):1081-1087.
【3】谢笑天, 郑萍, 朱伟明, 等.高效液相色谱法测定卷烟烟气总粒相物中的烟碱[J].分析化学, 2000, 28(9):1085-1087.
【4】储志兵, 周新光, 水恒福, 等.反相高效液相色谱法测定烟草中烟碱的含量[J].农业环境科学学报, 2006, 25(增刊2):751-754.
【5】储志兵, 周新光, 水恒福, 等.超声提取-高效液相色谱法测定烟草中烟碱含量[J].化工技术与开发, 2007, 36(2):46-48.
【6】MANCEAU F, FLINIAUX M A, JACQUIN-DUBREUIL A. A high performance liquid chromatographic procedure for the analysis of tobacco alkaloids——Application to the evaluation of tobacco alkaloids in plants and cell suspension cultures[J]. Phytochemical Analysis, 1992, 3(2):65-68.
【7】LEE J H, PATRA J K, SHIN H S. Analytical methods for determination of carbonyl compounds and nicotine in electronic No-Smoking aid refill solutions[J]. Analytical Biochemistry, 2020, 588:113470.
【8】MOHAMMED Z H. Determination of nicotine extracted from eggplant and green pepper by HPLC[J]. Baghdad Science Journal, 2019, 16(1):61-67.
【9】廖坤, 彭春鸣, 张翼, 等.气相色谱法测定烟草中的烟碱含量[J].烟草科技, 2003, 36(3):32-34.
【10】蔡君兰, 陈黎, 彭斌, 等.气相色谱法同时测定电子烟烟液中的烟碱、1, 2-丙二醇和丙三醇[J].中国烟草学报, 2016, 22(5):1-9.
【11】YANG S, SMETENA I, HUANG C. Determination of tobacco alkaloids by gas chromatography with nitrogen-phosphorus detection[J]. Analytical and Bioanalytical Chemistry, 2002, 373(8):839-843.
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