Rapid Screening of Illegal Added Lidocaine and Cinchocaine in Cosmetics by Surface-Enhanced Raman Spectrometry
摘 要
取样品约0.5 g,加入饱和氯化钠溶液0.5 mL,涡旋1 min。加入正己烷3 mL,超声提取3 min,离心30 s。取上清液2 mL,加入0.1 mol·L-1盐酸溶液0.5 mL,振荡30 s,离心15 s,取下层提取液作待测液。取纳米金溶胶150 μL,加入待测液50 μL以及1 mol·L-1氯化钠溶液50 μL,振荡10 s后供便携式拉曼光谱仪分析。结果显示,利多卡因和辛可卡因的表面增强拉曼特征峰组分别为505,670,1 081,1 254 cm-1和509,774,1 379,1 599 cm-1,可有效区分这两种药物和其他同类型局部麻醉药。利多卡因和辛可卡因的仪器检出限和方法检出限分别为0.10 mg·L-1和5 mg·kg-1。方法适用于固体乳膏、润肤水以及凝胶状精华等常见化妆品基质中利多卡因和辛可卡因2种局部麻醉药的快速筛查。
Abstract
The sample of 0.5 g was taken, and saturated sodium chloride solution of 0.5 mL was added. After swirling for 1 min, n-hexane of 3 mL was added, and the mixture obtained was extracted for 3 min by ultrasound, and centrifuged for 30 s. The supernatant of 2 mL was taken, and 0.1 mol·L-1 hydrochloric acid solution of 0.5 mL was added. After swirling for 30 s and centrifuging for 15 s, the lower layer of extract was collected. Colloridal gold nanoparticles of 150 μL was taken, and the lower layer extract of 50 μL and 1 mol·L-1 sodium chloride solution of 50 μL were added. The mixed solution was swirled for 10 s, and detected by portable Raman spectrometer. As shown by the results, the surfance-enhanced Raman characteristic peaks of lidocaine and cinchocaine were 505, 670, 1 081, 1 254 cm-1 and 509, 774, 1 379, 1 599 cm-1, and lidocaine and cinchocaine could be effectively distinguished from other local anesthetics by the above Raman characteristic peaks. Instrument detection limits of lidocaine and cinchocaine were 0.10 mg·L-1, and method detection limits were 5 mg·kg-1. The proposed method was suitable for rapid screening of the 2 local anesthtics (lidocaine and cinchocaine) in common cosmetic substrates, such as solid cream, lotion and gel essence.
中图分类号 O657.37 DOI 10.11973/lhjy-hx202302007
所属栏目 工作简报
基金项目 江苏省市场监督管理局科技计划项目(KJ207560)
收稿日期 2021/5/12
修改稿日期
网络出版日期
作者单位点击查看
联系人作者曹玲(clidc@sina.com)
备注方方,副主任药师,硕士,主要从事化妆品质量控制与安全评估工作
引用该论文: FANG Fang,ZHANG Miaomiao,ZHANG Zaiping,XIA Jingzhu,FENG Youlong,CAO Ling. Rapid Screening of Illegal Added Lidocaine and Cinchocaine in Cosmetics by Surface-Enhanced Raman Spectrometry[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2023, 59(2): 166~171
方方,张苗苗,张再平,夏婧竹,冯有龙,曹玲. 表面增强拉曼光谱法快速筛查化妆品中非法添加的利多卡因和辛可卡因[J]. 理化检验-化学分册, 2023, 59(2): 166~171
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【3】STOCKMANN S, SPIES E, GEHRING H, et al. Evaluation and application of a high performance liquid chromatographic method for prilocaine analysis in human plasma[J]. Clinical Laboratory, 2013,59(1/2):127-132.
【4】COUTO R O, CUBAYACHI C, LOPEZ R F V, et al. A simple and high-resolution HPLC-PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies[J]. Biomedical Chromatography, 2016,30(6):857-866.
【5】解大建,刘亚平,谢荣.气相色谱法测定人血浆中的利多卡因、布比卡因、普鲁卡因和丁卡因[J].北京医科大学学报, 1986,18(4):275-277.
【6】黄朝辉,蔡丹丹,陈仲益,等.UPLC-MS/MS测定延时类保健品中3种局麻药的含量[J].中国现代应用药学, 2014,31(1):99-102.
【7】刘晓锋,黄克建,林翠梧,等.在线固相萃取-液相色谱-质谱法测定血液中9种局部麻醉药[J].理化检验-化学分册, 2015,51(2):245-247.
【8】ZHAO L M, GAN L, ZHANG Y, et al. Matrix effect-free on-line pass-through cleanup procedure for the fast determination of local anesthetic drug by LC-MS/MS[J]. Journal of Chromatography B, 2019,1130/1131:121831.
【9】祝惠惠,曾勇明,陈启振,等.表面增强拉曼光谱法在食品中亚硝酸盐的快速筛查研究[J].中国食品添加剂, 2019,30(9):72-76.
【10】王昕,何坚,范贤光,等.基于表面增强拉曼光谱的合成色素专利蓝V的快速检测[J].光谱学与光谱分析, 2019,39(10):3205-3209.
【11】张宗绵,刘睿,徐敦明,等.Au@SiO2核壳结构-表面增强拉曼光谱原位检测食品中的酸性橙Ⅱ[J].化学学报, 2012,70(16):1686-1689.
【12】朱晓宇,艾施荣,彭雄鑫,等.茶叶中甲萘威农残表面增强拉曼光谱(SERS)结合快速前处理检测方法的建立[J].食品工业科技, 2019,40(10):242-247.
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【14】熊俊飞,吴瑞梅,郭平,等.芹菜中苯醚甲环唑农药残留表面增强拉曼光谱的快速检测[J].现代食品科技, 2016,32(4):283-287.
【15】周亚红,吴国萍,黄嘉婕.纳米银表面增强拉曼光谱法测定保健食品中非法添加的4种地平类降压药物[J].理化检验-化学分册, 2020,56(3):302-307.
【16】宋健.表面增强拉曼光谱法快速检测中成药中吡罗昔康[J].化学分析计量, 2020,29(2):49-53.
【17】陈蓉,周光明,罗丹,等.基于表面增强拉曼光谱技术检测化妆品中的香豆素[J].广州化工, 2019,47(4):84-88.
【18】陈宇宇,彭俊瑛,李光照,等.表面增强拉曼光谱法检测儿童化妆品中二噁烷含量的研究[J].光散射学报, 2015,27(4):316-319.
【19】国家质量监督检验检疫总局.进出口化妆品中利多卡因、丁卡因、辛可卡因的测定液相色谱-质谱/质谱法:SN/T 4147-2015[S].北京:中国标准出版社, 2015.
【20】陈志杰,马小卫,陈薪璇,等.利用表面增强拉曼光谱(SERS)技术快速检测水与尿液中舒芬太尼的研究[J].分析测试学报, 2020,39(9):1073-1078.
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