工业大麻中非兴奋剂成分大麻二酚检测方法的研究进展
Research Progress on Detection Methods of Cannabidiol as a Non-Doping Ingredient in Hemp
摘 要
大麻二酚(CBD)因其具有神经保护、镇痛、抗炎、抗氧化以及抗癫痫等药理作用而受到广泛的关注。然而随着CBD正式被列入化妆品禁用原料目录,其应用受到很大影响。同时,我国目前尚未制定CBD产品的相关法律法规、标准,跨境电商平台领域的CBD产品给监管带来极大的困难和挑战。基于此,介绍了CBD的药理作用及应用,综述了CBD检测方法的研究进展,以期为CBD的规范使用、CBD制品的规范开发提供新的思路(引用文献41篇)。
药理作用
检测方法
工业大麻
cannabidiol
pharmacological effect
detection method
hemp
Abstract
Cannabidiol (CBD) had received wide attention due to its pharmacological effects, including neuroprotective, analgesic, anti-inflammatory, anti-oxidant and anti-epileptic. However, as CBD was officially listed in the list of prohibited ingredients for cosmetics, the application of CBD had been greatly affected. Moreover, there were no relevant laws, regulations or standards for CBD products in China at present. The CBD products in the field of cross-border e-commerce platforms had brought great difficulties and challenges to the supervision. Based on these facts, the pharmacological effects and application of CBD were introduced, and the research progress of CBD detection methods was reviewed, in order to provide new insights for the standardized use of CBD and the standardized development of CBD products (41 ref. cited).
中图分类号 O65 DOI 10.11973/lhjy-hx202303019
所属栏目 综述
基金项目
收稿日期 2021/8/22
修改稿日期
网络出版日期
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备注徐晖,高级经济师,硕士,从事跨境商品质量安全风险评估工作
引用该论文: XU Hui,LU Zhengjie,LI Yi,WU Juan,CHEN Lingling. Research Progress on Detection Methods of Cannabidiol as a Non-Doping Ingredient in Hemp[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2023, 59(3): 367～372
徐晖,陆征杰,李翼,吴娟,陈玲玲. 工业大麻中非兴奋剂成分大麻二酚检测方法的研究进展[J]. 理化检验－化学分册, 2023, 59(3): 367～372
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参考文献
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【21】CRIPPA J A, DERENUSSON G N, FERRARI T B, et al. Neural basis of anxiolytic effects of cannabidiol (CBD) in generalized social anxiety disorder: A preliminary report[J]. Journal of Psychopharmacology (Oxford, England), 2011,25(1):121-130.
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【24】ROTH N, MOOSMANN B, AUWÄRTER V. Development and validation of an LC-MS/MS method for quantification of Δ9-tetrahydrocannabinolic acid A (THCA-a), THC, CBN and CBD in hair[J]. Journal of Mass Spectrometry: JMS, 2013,48(2):227-233.
【25】DA SILVA C P, DALPIAZ L P P, GERBASE F E, et al. Determination of cannabinoids in plasma using salting-out-assisted liquid-liquid extraction followed by LC-MS/MS analysis[J]. Biomedical Chromatography: BMC, 2020,34(12):4952.
【26】GURLEY B J, MURPHY T P, GUL W, et al. Content versus label claims in cannabidiol (CBD)-containing products obtained from commercial outlets in the state of Mississippi[J]. Journal of Dietary Supplements, 2020,17(5):599-607.
【27】TIPPARAT P, NATAKANKITKUL S, CHAMNIVIKAIPONG P, et al. Characteristics of cannabinoids composition of cannabis plants grown in Northern Thailand and its forensic application[J]. Forensic Science International, 2012,215(1/2/3):164-170.
【28】PATEL B, WENE D, FAN Z T. Qualitative and quantitative measurement of cannabinoids in cannabis using modified HPLC/DAD method[J]. Journal of Pharmaceutical and Biomedical Analysis, 2017,146:15-23.
【29】CIOLINO L A, RANIERI T L, TAYLOR A M. Commercial cannabis consumer products part 2:HPLC-DAD quantitative analysis of cannabis cannabinoids[J]. Forensic Science International, 2018,289:438-447.
【30】MADEJ K, KÓZKA G, WINIARSKI M, et al. A simple, fast, and green oil sample preparation method for determination of cannabidioloic acid and cannabidiol by HPLC-DAD[J]. Separations, 2020,7(4):60.
【31】PELLEGRINI M, MARCHEI E, PACIFICI R, et al. A rapid and simple procedure for the determination of cannabinoids in hemp food products by gas chromatography-mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis, 2005,36(5):939-946.
【32】王占良,张建丽,张亦农.气相色谱-质谱法同时分析运动营养品中的大麻酚、大麻二酚和Δ9-四氢大麻酚[J].中国运动医学杂志, 2015,34(4):398-403.
【33】PAUL R, WILLIAMS R, HODSON V, et al. Detection of cannabinoids in hair after cosmetic application of hemp oil[J]. Scientific Reports, 2019,9:2582.
【34】CIOLINO L A, RANIERI T L, TAYLOR A M. Commercial cannabis consumer products part 1:GC-MS qualitative analysis of cannabis cannabinoids[J]. Forensic Science International, 2018,289:429-437.
【35】MENG Q F, BUCHANAN B, ZUCCOLO J, et al. A reliable and validated LC-MS/MS method for the simultaneous quantification of 4 cannabinoids in 40 consumer products[J]. PLoS One, 2018,13(5):0196396.
【36】NEMEŠKALOVÁ A, HÁJKOVÁ K, MIKUL L, et al. Combination of UV and MS/MS detection for the LC analysis of cannabidiol-rich products[J]. Talanta, 2020,219:121250.
【37】周莹,陈念念,韩丽,等.UPLC-MS/MS同时测定四种食品基质中痕量四氢大麻酚、大麻二酚和大麻酚[J].现代食品科技, 2019,35(12):315-321.
【38】王全林,张爱芝.超高效液相色谱-串联质谱法测定火麻食品中特征大麻酚[J].理化检验-化学分册, 2013,49(6):720-724.
【39】张爱芝,王全林,莫世杰.超高效液相色谱-串联质谱法同时测定食用油中痕量的δ-9-四氢大麻酚、大麻酚和大麻二酚[J].色谱, 2010,28(11):1015-1019.
【40】LEE J H, MIN A Y, HAN J H, et al. Development and validation of LC-MS/MS method with QuEChERS clean-up for detecting cannabinoids in foods and dietary supplements[J]. Food Additives & Contaminants Part A, 2020,37(9):1413-1424.
【2】SALES A J, FOGACA M V, SARTIM A G, et al. Cannabidiol induces rapid and sustained antidepressant-like effects through increased BDNF signaling and synaptogenesis in the prefrontal cortex[J]. Molecular Neurobiology, 2019,56(2):1070-1081.
【3】MORI M A, MEYER E, SOARES L M, et al. Cannabidiol reduces neuroinflammation and promotes neuroplasticity and functional recovery after brain ischemia[J]. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 2017,75:94-105.
【4】BORGES R S, BATISTA J J, VIANA R B, et al. Understanding the molecular aspects of tetrahydrocannabinol and cannabidiol as antioxidants[J]. Molecules (Basel, Switzerland), 2013,18(10):12663-12674.
【5】ATALAY S, JAROCKA-KARPOWICZ I, SKRZYDLEWSKA E. Antioxidative and anti-inflammatory properties of cannabidiol[J]. Antioxidants (Basel, Switzerland), 2019,9(1):21.
【6】RAMER R, SCHWARZ R, HINZ B. Modulation of the endocannabinoid system as a potential anticancer strategy[J]. Frontiers in Pharmacology, 2019,10:430.
【7】ELBAZ M, NASSER M W, RAVI J, et al. Modulation of the tumor microenvironment and inhibition of EGF/EGFR pathway: Novel anti-tumor mechanisms of cannabidiol in breast cancer[J]. Molecular Oncology, 2015,9(4):906-919.
【8】HINZ B, RAMER R. Anti-tumour actions of cannabinoids[J]. British Journal of Pharmacology, 2019,176(10):1384-1394.
【9】DAS S, KAUL K, MISHRA S, et al. Cannabinoid signaling in cancer[J]. Advances in Experimental Medicine and Biology, 2019,1162:51-61.
【10】蒋鸿雁,张瑞林,曹艳,等.大麻二酚在医学上的应用前景[J].昆明医科大学学报, 2021,42(2):147-152.
【11】陈宝军,史利军.一种纳米水溶性大麻二酚美容浸膏及制备方法:112741779A[P]. 2021-05-04.
【12】张可,谭昕,于朝晖,等.大麻二酚或大麻提取物在制备美白产品中的用途:107982080A[P]. 2018-05-04.
【13】陈宝军,史利军. 一种含大麻二酚的保湿身体乳: 112933008A[P]. 2021-06-11.
【14】OLÁH A, TÓTH B I, BORBÍRÓ I, et al. Cannabidiol exerts sebostatic and antiinflammatory effects on human sebocytes[J]. The Journal of Clinical Investigation, 2014,124(9):3713-3724.
【15】WILKINSON J D, WILLIAMSON E M. Cannabinoids inhibit human keratinocyte proliferation through a non-CB1/CB2 mechanism and have a potential therapeutic value in the treatment of psoriasis[J]. Journal of Dermatological Science, 2007,45(2):87-92.
【16】VINCENZI C, TOSTI A. Efficacy and tolerability of a shampoo containing broad-spectrum cannabidiol in the treatment of scalp inflammation in patients with mild to moderate scalp psoriasis or seborrheic dermatitis[J]. Skin Appendage Disorders, 2020,6(6):355-361.
【17】JAROCKA-KARPOWICZ I, BIERNACKI M, WROЙSKI A, et al. Cannabidiol effects on phospholipid metabolism in keratinocytes from patients with psoriasis vulgaris[J]. Biomolecules, 2020,10(3):367.
【18】叶程龙,余伟,莫斯喻,等.大麻二酚对应激大鼠肺部损伤的预防保护作用[J].广东药科大学学报, 2019,35(2):234-237.
【19】RIBEIRO A, ALMEIDA V I, COSTOLA-DE-SOUZA C, et al. Cannabidiol improves lung function and inflammation in mice submitted to LPS-induced acute lung injury[J]. Immunopharmacology and Immunotoxicology, 2015,37(1):35-41.
【20】LINGE R, JIMÉNEZ-SÁNCHEZ L, CAMPA L, et al. Cannabidiol induces rapid-acting antidepressant-like effects and enhances cortical 5-HT/glutamate neurotransmission: Role of 5-HT1A receptors[J]. Neuropharmacology, 2016,103:16-26.
【21】CRIPPA J A, DERENUSSON G N, FERRARI T B, et al. Neural basis of anxiolytic effects of cannabidiol (CBD) in generalized social anxiety disorder: A preliminary report[J]. Journal of Psychopharmacology (Oxford, England), 2011,25(1):121-130.
【22】IBRAHIM E A, GUL W, GUL S W, et al. Determination of acid and neutral cannabinoids in extracts of different strains of cannabis sativa using GC-FID[J]. Planta Medica, 2018,84(4):250-259.
【23】孙维来,郑晓雨,赵彦彪,等.基于超高效液相色谱-质谱联用技术对大麻植物中3种成分及化学表型分析[J].分析化学, 2017,45(7):1052-1058.
【24】ROTH N, MOOSMANN B, AUWÄRTER V. Development and validation of an LC-MS/MS method for quantification of Δ9-tetrahydrocannabinolic acid A (THCA-a), THC, CBN and CBD in hair[J]. Journal of Mass Spectrometry: JMS, 2013,48(2):227-233.
【25】DA SILVA C P, DALPIAZ L P P, GERBASE F E, et al. Determination of cannabinoids in plasma using salting-out-assisted liquid-liquid extraction followed by LC-MS/MS analysis[J]. Biomedical Chromatography: BMC, 2020,34(12):4952.
【26】GURLEY B J, MURPHY T P, GUL W, et al. Content versus label claims in cannabidiol (CBD)-containing products obtained from commercial outlets in the state of Mississippi[J]. Journal of Dietary Supplements, 2020,17(5):599-607.
【27】TIPPARAT P, NATAKANKITKUL S, CHAMNIVIKAIPONG P, et al. Characteristics of cannabinoids composition of cannabis plants grown in Northern Thailand and its forensic application[J]. Forensic Science International, 2012,215(1/2/3):164-170.
【28】PATEL B, WENE D, FAN Z T. Qualitative and quantitative measurement of cannabinoids in cannabis using modified HPLC/DAD method[J]. Journal of Pharmaceutical and Biomedical Analysis, 2017,146:15-23.
【29】CIOLINO L A, RANIERI T L, TAYLOR A M. Commercial cannabis consumer products part 2:HPLC-DAD quantitative analysis of cannabis cannabinoids[J]. Forensic Science International, 2018,289:438-447.
【30】MADEJ K, KÓZKA G, WINIARSKI M, et al. A simple, fast, and green oil sample preparation method for determination of cannabidioloic acid and cannabidiol by HPLC-DAD[J]. Separations, 2020,7(4):60.
【31】PELLEGRINI M, MARCHEI E, PACIFICI R, et al. A rapid and simple procedure for the determination of cannabinoids in hemp food products by gas chromatography-mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis, 2005,36(5):939-946.
【32】王占良,张建丽,张亦农.气相色谱-质谱法同时分析运动营养品中的大麻酚、大麻二酚和Δ9-四氢大麻酚[J].中国运动医学杂志, 2015,34(4):398-403.
【33】PAUL R, WILLIAMS R, HODSON V, et al. Detection of cannabinoids in hair after cosmetic application of hemp oil[J]. Scientific Reports, 2019,9:2582.
【34】CIOLINO L A, RANIERI T L, TAYLOR A M. Commercial cannabis consumer products part 1:GC-MS qualitative analysis of cannabis cannabinoids[J]. Forensic Science International, 2018,289:429-437.
【35】MENG Q F, BUCHANAN B, ZUCCOLO J, et al. A reliable and validated LC-MS/MS method for the simultaneous quantification of 4 cannabinoids in 40 consumer products[J]. PLoS One, 2018,13(5):0196396.
【36】NEMEŠKALOVÁ A, HÁJKOVÁ K, MIKUL L, et al. Combination of UV and MS/MS detection for the LC analysis of cannabidiol-rich products[J]. Talanta, 2020,219:121250.
【37】周莹,陈念念,韩丽,等.UPLC-MS/MS同时测定四种食品基质中痕量四氢大麻酚、大麻二酚和大麻酚[J].现代食品科技, 2019,35(12):315-321.
【38】王全林,张爱芝.超高效液相色谱-串联质谱法测定火麻食品中特征大麻酚[J].理化检验-化学分册, 2013,49(6):720-724.
【39】张爱芝,王全林,莫世杰.超高效液相色谱-串联质谱法同时测定食用油中痕量的δ-9-四氢大麻酚、大麻酚和大麻二酚[J].色谱, 2010,28(11):1015-1019.
【40】LEE J H, MIN A Y, HAN J H, et al. Development and validation of LC-MS/MS method with QuEChERS clean-up for detecting cannabinoids in foods and dietary supplements[J]. Food Additives & Contaminants Part A, 2020,37(9):1413-1424.
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