高效液相色谱法测定右旋雷贝拉唑钠的对映体纯度

吉丹丹; 张玲; 谭淑珍; 薛琳

doi:10.11973/lhjy-hx201507030

高效液相色谱法测定右旋雷贝拉唑钠的对映体纯度

长沙理工大学化学与生物工程学院, 长沙 410114

详细信息

作者简介:
吉丹丹(1991-),女,湖南长沙人,硕士研究生,主要研究方向为药物合成及分析.

中图分类号: O657.7
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出版历程
- 收稿日期: 2014-07-20
- 刊出日期: 2015-07-17

Determination of Enantiomeric Purity of R-Rabeprazole Sodium by HPLC

摘要

摘要: 采用高效液相色谱法测定右旋雷贝拉唑钠对映体的纯度.以α1-酸性糖蛋白键合硅胶为填充剂的CHIRALPAK-AGP手性柱为固定相,用乙腈-pH 6.0磷酸盐(1+9)混合溶液为流动相进行洗脱,用紫外检测器进行测定,检测波长为285 nm.左旋雷贝拉唑钠的质量浓度在38.4~576 μg·L-1范围内与其峰面积呈线性关系,检出限(3S/N)为0.2 ng,测定下限(10S/N)为0.56 ng.加标回收率在91.1%~102%之间,测定值的相对标准偏差在2.4%~4.3%之间.
- 高效液相色谱法 /
- 右旋雷贝拉唑钠 /
- 对映体纯度
Abstract: A HPLC method was developed for the determination of enantiomeric purity of R-rabeprazole sodium. A chiral column (CHIRALPAK-AGP) packed with α1-acid glycoprotein immobilized on silica gel was used as the stationary phase,with a mixed solution of acetonitrile and phosphate buffer (pH 6.0) as the mobile phase for elution. UV detection at the wavelength of 285 nm was utilized. Linear relationship between the peak area and the mass concentration of S-rabeprazole sodium were found in the range of 38.4-576 μg·L-1 with detection limit (3S/N) of 0.2 ng and the lower limit of determination (10S/N) of 0.56 ng. The recovery rates measured by standard addition method were in the range of 91.1%-102%,and RSD were in the range of 2.4%-4.3%.
- HPLC /
- R-Rabeprazole sodium /
- Enantiomeric purity

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参考文献(14)

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高效液相色谱法测定右旋雷贝拉唑钠的对映体纯度

作者简介: 吉丹丹(1991-),女,湖南长沙人,硕士研究生,主要研究方向为药物合成及分析.

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出版历程

Determination of Enantiomeric Purity of R-Rabeprazole Sodium by HPLC

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目录

作者简介:
吉丹丹(1991-),女,湖南长沙人,硕士研究生,主要研究方向为药物合成及分析.