Application of In Vitro Skin Allergy-Amino Acid Derivative Reactivity Assay in Preliminary Screening of Sensitization of Sunscreens
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摘要: 采用氨基酸衍生物反应试验(ADRA)对20种化学防晒剂的致敏性进行评价。利用半胱氨酸衍生物N-[2-(1-萘基)乙酰基]-L-半胱氨酸(NAC)和赖氨酸衍生物α-N-[2-(1-萘基)乙酰基]-L-赖氨酸(NAL)模拟皮肤蛋白,与化学防晒剂共同反应,通过高效液相色谱法测定20种化学防晒剂的氨基酸衍生物消耗百分比,并对皮肤致敏性进行预测。通过比较ADRA预测结果与欧盟消费者安全科学委员会(SCCS)出示的化合物评估报告的一致性,判断方法的可行性。结果表明,3-亚苄基樟脑等19种化学防晒剂预测结果为阴性,二苯酮-3预测结果为阳性。ADRA可作为动物试验体外替代方法,简便、快捷地对化合物的皮肤致敏性进行预测和筛选。
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关键词:
- 氨基酸衍生物反应试验(ADRA) /
- 高效液相色谱法 /
- 皮肤致敏性 /
- 化学防晒剂
Abstract: The sensitization of 20 chemical sunscreens was evaluated using amino acid derivative reactivity assay (ADRA). Cysteine derivative N-[2-(1-naphthyl) acetyl]-L-cysteine (NAC) and lysine derivative α-N-[2-(1-naphthyl) acetyl]-L-lysine (NAL) were used to mimic skin proteins, which reacted with chemical sunscreens. The amino acid derivative depletion percentage of 20 chemical sunscreens were determined by high performance liquid chromatography, and then their skin sensitization were predicted. The feasibility of ADRA method was judged by comparing the consistency of ADRA prediction results with the compound evaluation reports produced by European Scientific Committee on Consumer Safety (SCCS). It was shown that 19 chemical sunscreens such as 3-benzylidene camphor were predicted to be negative, and benzophenone-3 was predicted to be positive. ADRA could be used as an alternative in vitro method for animal experiments to predict and screen the skin sensitization of compounds simply and quickly. -
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[1] 杜顺晶,房军,金银龙.皮肤变态反应体外替代方法研究进展[J].环境与健康杂志, 2009,26(5):462-464. [2] 洪丽玲,周庆云.皮肤致敏反应体外替代方法的研究现状[J].实验动物科学, 2012,29(6):60-63. [3] 喻才正,周婷,黄倩,等.动物皮肤致敏试验方法的研究进展[J].华中科技大学学报(医学版), 2016,45(2):224-228. [4] Organization for Economic Cooperation and Development. In vitro skin sensitisation: ARE-Nrf2 luciferase test method: TG 442D[S]. Paris: Organization for Economic Cooperation and Development, 2015.
[5] COTTREZ F, BOITEL E, AURIAULT C, et al. Genes specifically modulated in sensitized skins allow the detection of sensitizers in a reconstructed human skin model. Development of the SENS-IS assay[J]. Toxicology in Vitro, 2015,29(4):787-802.
[6] 李晨,沙毅杰,霍倩,等.人源THP-1细胞系活化试验在化妆品皮肤致敏性评价中的应用研究[J].现代预防医学, 2022,49(2):349-353. [7] HUPPERT C, PARIS C, LANGONNÉ I, et al. Activation of T cells by dendritic cells exposed to a reference sensitizer: Towards a promising model to assess the allergenic potential of chemicals[J]. Contact Dermatitis, 2018,79(2):67-75.
[8] Organization for Economic Cooperation and Development. In chemico skin sensitisation: Assays addressing the adverse outcome pathway key event on covalent binding to proteins: TG 442C[S]. Paris: Organization for Economic Cooperation and Development, 2019.
[9] 耿雪,王小兵,魏霞,等.一种新型in silico模型在化妆品原料皮肤致敏性初筛中的应用[J].香料香精化妆品, 2020(2):27-32. [10] YAMAMOTO Y, TAHARA H, USAMI R, et al. A novel in chemico method to detect skin sensitizers in highly diluted reaction conditions[J]. Journal of Applied Toxicology, 2015,35(11):1348-1360.
[11] 所雅琼,罗飞亚,张凤兰,等.皮肤致敏替代方法和整合策略研究进展及思考[J].香料香精化妆品, 2021(5):96-102. [12] 杨婷婷,易路遥,王绎,等.采用DPRA替代方法评价3种香料的皮肤致敏性[J].日用化学工业, 2021,51(5):438-442.
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