HPLC Determination of SudanⅠ-Ⅳ in Food with Molecularly Imprinted Solid Phase Extraction
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摘要: 以苏丹红Ⅰ为模板分子,甲基丙烯酸为功能单体合成了苏丹红分子印迹聚合物,作为固相萃取吸附剂,用于食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的测定。样品经乙腈提取,所得提取液分子印迹固相萃取柱,然后用乙醇-正己烷(1+1)溶液作洗脱剂将苏丹红洗下,收集洗出液供高效液相色谱测定。结果表明:聚合物对印迹分子具有很好的亲和性和特异选择性。方法的线性范围为0.5~15 mg·L-1,检出限(3S/N)为4 μg·kg-1。以辣椒粉和辣椒酱为基体,在3个浓度水平下做加标回收试验,回收率在70.3%~85.5%之间,测定值的相对标准偏差(n=5)低于6.0%。Abstract: The molecularly imprinted polymer of Sudan red was synthesized with Sudan Ⅰ as template molecule and methacrylic acid as functional monomer, and it was used to extract Sudan Ⅰ-Ⅳ from food as adsorbent in solid phase extraction column. The sample was extracted with acetonitrile, and the extract obtained were purified on MIP-SPE column. Sudan Ⅰ-Ⅳ adsorbed on the imprinted polymer were eluted with ethanol-n-hexane (1+1) mixture, which were collected and used for HPLC determination. It was found that the imprinted polymer had good affinity and excellent selectivity for SudanⅠ-Ⅳ. Linearity range for the determination was found between 0.5-15 mg·L-1, and detection limit (3S/N) was 4 μg·kg-1. Using chili powder and chili sauce as matrixes, test for recovery was made at 3 concentration levels, values of recovery found were in the range of 70.3%-85.5% with RSD′s (n=5) less than 6.0%.
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Keywords:
- HPLC /
- Sudan Ⅰ-Ⅳ /
- Molecularly imprinted polymer /
- Solid phase extraction
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